Enhancement of CDP-DG:Inositol Transferase Activity in src- and erbB2-Transformed Cells

• Published in: Experimental cell research Vol. 212; no. 1; pp. 151 - 154
• Main Authors: Imoto, Masaya, Taniguchi, Yoshiko, Fujiwara, Hiroaki, Umezawa, Kazuo
• Format: Journal Article
• Language: English
• Published: Orlando, FL Elsevier Inc 01.05.1994; Elsevier
• Subjects: 1-Phosphatidylinositol 4-Kinase; 3T3 Cells; Animals; Biological and medical sciences; CDP-Diacylglycerol-Inositol 3-Phosphatidyltransferase; Cell Line, Transformed; Cell metabolism, cell oxidation; Cell physiology; Cell Transformation, Neoplastic; Fundamental and applied biological sciences. Psychology; Genes, src - genetics; Hydroquinones - pharmacology; Mice; Molecular and cellular biology; Oncogene Proteins, Viral - genetics; Phosphatidylinositols - biosynthesis; Phosphotransferases (Alcohol Group Acceptor) - metabolism; Protein-Tyrosine Kinases - antagonists & inhibitors; Receptor, ErbB-2; Transferases (Other Substituted Phosphate Groups) - metabolism; Type C Phospholipases - metabolism; Phosphatidylinositol; Regulation(control); Cell line; Enzymatic activity; Enzyme; Transformed cell; Hydrolases; Esterases; Phosphoric diester hydrolases; Phospholipase C
• ISSN: 0014-4827; 1090-2422
• DOI: 10.1006/excr.1994.1129

Phosphatidylinositol (PI) synthesis was activated in Rous sarcoma virus-infected NIH3T3 or activated erbB2-transformed NIH3T3 cells. The in vitro activity of CDP-DG:inositol transferase prepared from these cells was also higher than that from normal parent NIH3T3 cells, although phospholipase C and PI kinase activities were not significantly different among these cells. A tyrosine kinase inhibitor, erbstatin, inhibited the PI synthesis in cultured cells, suggesting that Src and ErbB2-associated tyrosine kinases are involved in activation of CDP-DG:inositol transferase in these cell lines.