Analysis of vitamin K1 in fruits and vegetables using accelerated solvent extraction and liquid chromatography tandem mass spectrometry with atmospheric pressure chemical ionization

• Published in: Food chemistry Vol. 192; pp. 402 - 408
• Main Authors: Jäpelt, Rie Bak, Jakobsen, Jette
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.02.2016
• Subjects: Accelerated solvent extraction; APCI; Atmospheric Pressure; Chromatography, Liquid - methods; Fruit - chemistry; Fruits and vegetables; LC–MS/MS; Solvents - chemistry; Tandem Mass Spectrometry - methods; Vegetables - chemistry; Vitamin K 1 - chemistry; Vitamin K1; Vitamin K1; Fruits and vegetables; Accelerated solvent extraction; APCI; LC–MS/MS; Vitamin K
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2015.06.111

•Development of analytical method to study vitamin K1 in fruits and vegetables.•Extraction by accelerated solvent extraction and solid phase extraction.•Quantification by LC–APCI–MS/MS with vitamin K1-d7 as internal standard.•Precision was 6.2%, recovery 90–120%, and LOQ 0.05μg/100g fresh weight. The objective of this study was to develop a rapid, sensitive, and specific analytical method to study vitamin K1 in fruits and vegetables. Accelerated solvent extraction and solid phase extraction was used for sample preparation. Quantification was done by liquid chromatography tandem mass spectrometry with atmospheric pressure chemical ionization in selected reaction monitoring mode with deuterium-labeled vitamin K1 as an internal standard. The precision was estimated as the pooled estimate of three replicates performed on three different days for spinach, peas, apples, banana, and beetroot. The repeatability was 5.2% and the internal reproducibility was 6.2%. Recovery was in the range 90–120%. No significant difference was observed between the results obtained by the present method and by a method using the same principle as the CEN-standard i.e. liquid–liquid extraction and post-column zinc reduction with fluorescence detection. Limit of quantification was estimated to 0.05μg/100g fresh weight.