new HPLC analytical method to study fungal caffeine metabolism

A simple and rapid HPLC method has been developed to analyse all the methylxanthines that can be produced by N-demethylation of 1,3,7-trimethylxanthine (caffeine). This method is particularly suitable to study caffeine metabolism of a filamentous fungus (Aspergillus sp V12A25) cultivated in a synthe...

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Bibliographic Details
Published inBiotechnology techniques Vol. 12; no. 5; pp. 359 - 362
Main Authors Denis, S, Augur, C, Marin, B, Roussos, S
Format Journal Article
LanguageEnglish
Published London Chapman and Hall 01.05.1998
Subjects
Aspergillus
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Fundamental and applied biological sciences. Psychology
methylxanthines
Mission oriented research
Physiology and metabolism
xanthines
Fungi
Aspergillus
Demethylation
Analysis method
Metabolite
HPLC chromatography
Xanthine derivatives
Fungi Imperfecti
Metabolism
Thallophyta
Caffeine
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Summary:A simple and rapid HPLC method has been developed to analyse all the methylxanthines that can be produced by N-demethylation of 1,3,7-trimethylxanthine (caffeine). This method is particularly suitable to study caffeine metabolism of a filamentous fungus (Aspergillus sp V12A25) cultivated in a synthetic liquid medium containing caffeine as the sole source of nitrogen.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0951-208X
1573-6784
DOI:10.1023/A:1008866113065