Allyl Dibenzoylmethane Derivative: Antimelanoma Activity and Study of Its Molecular Mechanism of Interaction with DNA

In this study, we assessed the cytotoxic effect of 1,3-diphenyl-2-allyl-1,3-propanedione (DPAP) on B16F10 and Tm5 melanoma cells and investigated its interaction with DNA (deoxyribonucleic acid) using optical tweezers. The compound showed to be effective against B16F10 cells with selectivity index (...

Full description

Saved in:
Bibliographic Details
Published inJournal of the Brazilian Chemical Society Vol. 32; no. 7; pp. 1491 - 1497
Main Authors Baeta, Jefferson, Andrade, Ulisses, Oliveira, Rayane, Oliveira, Leandro, Santos, Anésia, Diaz-Muñoz, Gaspar, Rocha, Márcio, Diaz, Marisa
Format Journal Article
LanguageEnglish
Published Sociedade Brasileira de Química 01.07.2021
Subjects
CHEMISTRY, MULTIDISCIPLINARY
Tm5 cells
optical tweezers
DPAP
DNA
B16F10 cells
dacarbazine
Online AccessGet full text

Cover

More Information
Summary:In this study, we assessed the cytotoxic effect of 1,3-diphenyl-2-allyl-1,3-propanedione (DPAP) on B16F10 and Tm5 melanoma cells and investigated its interaction with DNA (deoxyribonucleic acid) using optical tweezers. The compound showed to be effective against B16F10 cells with selectivity index (SI) of 7.92 and interaction to the outer surface of the double helix, probably at the minor groove of DNA. This likely induced a bending deformation of the polymer chain, decreasing persistence length, and indicated that each DPAP molecule occupies a single base pair upon binding to the double helix.
ISSN:0103-5053
1678-4790
DOI:10.21577/0103-5053.20210048