Novel transdermal photodynamic therapy using ATX-S10·Na(II) induces apoptosis of synovial fibroblasts and ameliorates collagen antibody-induced arthritis in mice

• Published in: Rheumatology international Vol. 26; no. 8; pp. 717 - 725
• Main Authors: MIYAZAWA, S, NISHIDA, K, KOMIYAMA, T, NAKAE, Y, TAKEDA, K, YORIMITSU, M, KITAMURA, A, KUNISADA, T, OHTSUKA, A, INOUE, H
• Format: Journal Article
• Language: English
• Published: Berlin Springer 01.06.2006; Springer Nature B.V
• Subjects: Arthritis; Biological and medical sciences; Cells; Cellular biology; Diseases of the osteoarticular system; Diseases of the skin. Cosmetics; Inflammatory joint diseases; Medical sciences; Photodynamic therapy; Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects); Photodynamic therapy; Antibody; Rodentia; Diseases of the osteoarticular system; Rheumatology; Autoimmune disease; ATX-SlO-Na(II); Inflammatory joint disease; Vertebrata; Chronic; Mammalia; Treatment; Mouse; Animal; Rheumatoid arthritis; Fibroblast; Apoptosis
• ISSN: 0172-8172; 1437-160X
• DOI: 10.1007/s00296-005-0052-9

We aimed to test the effect of transdermal photodynamic therapy (PDT) on synovial proliferation in vitro and in vivo, using a novel photosensitizer, ATX-S10·Na(II). Synovial fibroblasts were obtained from patients with RA (RASF). Cell viability with or without PDT was determined by MTT assay. Cell morphology was examined by light and transmission electron microscopy. DNA fragmentation was labeled by TUNEL stain. Collagen antibody-induced arthritis (CAIA) was induced in DBA/1 mice, and the effects of transdermal PDT were evaluated by clinical and histological examination. PDT showed drug concentration-dependent and laser dose-dependent cytotoxicity on RASF. TUNEL stain and TEM study revealed the induction of apoptotic cell death of RASF. Transdermal PDT significantly reduced clinical arthritis and synovial inflammation in this model of arthritis. These results suggest that transdermal PDT using ATX-S10·Na(II) might be a novel less invasive treatment strategy for small joint arthritis and tenosynovitis.[PUBLICATION ABSTRACT]