Differentiation of soybean mosaic virus isolates by one-dimensional trypsin peptide maps immunoblotted with monoclonal antibodies

• Published in: Phytopathology Vol. 79; no. 11; pp. 1261 - 1265
• Main Authors: Hill, J.H. (Iowa State University, Ames), Benner, H.I, Permar, T.A, Bailey, T.B, Andrews, R.E. Jr, Durand, D.P, Van Deusen, R.A
• Format: Journal Article
• Language: English
• Published: St. Paul, MN American Phytopathological Society 01.11.1989
• Subjects: ANTICORPS MONOCLONAL; ANTICUERPOS MONOCLONALES; ANTIGENE; ANTIGENOS; Biological and medical sciences; CARTOGRAFIA; CARTOGRAPHIE; Fundamental and applied biological sciences. Psychology; GLYCINE MAX; PEPTIDE; PEPTIDOS; Phytopathology. Animal pests. Plant and forest protection; Plant viruses and viroids; Systematics. Structure, properties and multiplication. Genetics; TECHNIQUE IMMUNOENZYMATIQUE; TECHNIQUE IMMUNOLOGIQUE; TECNICAS INMUNOENZIMATICAS; TECNICAS INMUNOLOGICAS; TRIPSINA; TRYPSINE; VIRUS DE LAS PLANTAS; VIRUS DES VEGETAUX; Soybean mosaic virus; Taxonomy; Phytopathogen; Serology; Monoclonal antibody; Epidemiology; Infection; Virus; Peptide map; Antigenic variation; Viral disease; Immunoblotting assay; Coat protein; Potyvirus; Differentiation
• ISSN: 0031-949X; 1943-7684
• DOI: 10.1094/Phyto-79-1261

Twelve monoclonal antibodies were used to differentiate 11 isolates of soybean mosaic virus by immunoblot analysis of one-dimensional trypsin peptide maps of the virion capsid protein. Although all isolates showed unique patterns that reflected epitopic specificity of the viral coat protein, the virus isolates formed three distinct groups. These results suggest potential for using this approach to study antigenic drift and plant virus epidemiology