Bioconversion of vitamin D3 to calcifediol by using resting cells of Pseudonocardia sp

OBJECTIVES: Resting cells of Pseudonocardia sp. KCTC 1029BP were used for the bioconversion of vitamin D₃ to calcifediol which is widely used to treat osteomalacia and is industrially produced by chemical synthesis. RESULTS: To obtain the maximum bioconversion yield of calcifediol by the microbial c...

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Published inBiotechnology letters Vol. 37; no. 9; pp. 1895 - 1904
Main Authors Kang, Dae-Jung, Im, Jong-Hyuk, Kang, Jae-Hoon, Kim, Kyoung Heon
Format Journal Article
LanguageEnglish
Published Dordrecht Springer Netherlands 01.09.2015
Subjects
25-hydroxycholecalciferol
Actinobacteria - isolation & purification
Actinobacteria - metabolism
aeration
agitation
Applied Microbiology
Biochemistry
Biomedical and Life Sciences
Bioreactors
Biotechnology
biotransformation
Calcifediol - biosynthesis
cholecalciferol
Cholecalciferol - metabolism
fermenters
Industrial Microbiology
Life Sciences
Microbiology
Original Research Paper
osteomalacia
Pseudonocardia
Soil Microbiology
synthesis
temperature
Bioconversion
Calcifediol
Plackett–Burman design
Central composite design
Vitamin D
sp
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Summary:OBJECTIVES: Resting cells of Pseudonocardia sp. KCTC 1029BP were used for the bioconversion of vitamin D₃ to calcifediol which is widely used to treat osteomalacia and is industrially produced by chemical synthesis. RESULTS: To obtain the maximum bioconversion yield of calcifediol by the microbial conversion of vitamin D₃, a two-step optimization process was used, including the Plackett–Burman and the central composite designs. Six variables, namely agitation speed, aeration rate, resting cell concentration, vitamin D₃ concentration, temperature, and pH, were monitored. Of these, aeration rate, resting cell concentration, and temperature were selected as key variables for calcifediol production and were optimized using the central composite design. Optimal bioconversion conditions obtained were as follows: aeration rate of 0.2 vvm, resting cell concentration of 4.7 % w/v, and temperature of 33 °C. CONCLUSION: Using the optimal conditions, 356 mg calcifediol l⁻¹ was obtained with a bioconversion yield of 59.4 % in a 75 l fermentor. These are the highest values reported to date.
Bibliography:http://dx.doi.org/10.1007/s10529-015-1862-9
ObjectType-Article-1
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ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-015-1862-9