Determination of foscarnet (trisodium phosphonoformate) in pharmaceutical preparations by high-performance liquid chromatography with ultraviolet detection

• Published in: Biomedical chromatography Vol. 20; no. 10; pp. 1024 - 1027
• Main Authors: García, Javier, Márquez, Apolonia, Ruiz, Rosa, López, Luis F., Claro, Carmen, Lucero, María Jesús
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.10.2006
• Subjects: antiviral agent; Chromatography, High Pressure Liquid - methods; foscarnet; Foscarnet - analysis; isoosmotic sodium chloride solution; Pharmaceutical Preparations - analysis; Reproducibility of Results; Spectrophotometry, Ultraviolet - methods; trisodium phosphonophormate
• ISSN: 0269-3879; 1099-0801
• DOI: 10.1002/bmc.629

An isocratic high‐performance liquid chromatographic method with UV detection has been developed and validated for the quantitative determination of foscarnet in isoosmotic sodium chloride aqueous solution. The mobile phase consisted of mixture of methanol:water (30:70 v/v), containing 1 mm tetrahexylammonium hydrogen sulphate at pH 5.80. The analyte was separated on a reversed‐phase C18 column packed with 4 µm spherical particles of octadecylsilane. Hydrochlorothiazide was used as internal standard. UV detection at 232 nm allowed a quantification limit of 50 µg/mL. The assay was linear from 50 to 4000 µg/mL. The coefficient of variation was ≤2.52% for intra‐assay precision and ≤3.49% for inter‐assay precision. The deviation from the nominal value ranged from −0.57 to 0.47% for the same‐day accuracy and from −0.75 to 3.06% for day‐to‐day accuracy. Copyright © 2006 John Wiley & Sons, Ltd.