Simultaneous quantification of Fluticasone propionate and Olopatadine hydrochloride by High‐performance thin‐layer chromatography

• Published in: Separation science plus Vol. 6; no. 9
• Main Authors: Patel, Bhoomi, Patel, Satish
• Format: Journal Article
• Language: English
• Published: 01.09.2023
• Subjects: fluticasone propionate; high‐performance thin‐layer chromatography; olopatadine HCl; specificity; validation
• ISSN: 2573-1815; 2573-1815
• DOI: 10.1002/sscp.202300070

High‐performance thin‐layer chromatography has the capacity to run many samples simultaneously and reduced solvent usage. It offers a number of advantages that can save both time and money. The simultaneous quantification of fluticasone propionate and olopatadine hydrochloride in nasal spray has been developed and validated using a specific, precise, accurate, and economical high‐performance thin‐layer chromatography technique. Methanol:water:glacial acetic acid (8:2:0.05, v/v/v) was used as the mobile phase during the separation, which took place on a stationary phase of pre‐coated silica gel aluminum plate 60 F254. At 232 nm, separated components were densitometrically measured. The method was validated for linearity and range, precision, reproducibility, specificity, accuracy, the limit of detection, and the limit of quantification as per the International Council for Harmonization Q2(R1) guideline. Fluticasone propionate and olopatadine hydrochloride regression coefficients (R2) were found to be 0.9996 and 0.9991, accordingly. Fluticasone propionate and olopatadine hydrochloride percentage recoveries were measured to be 101.6 ± 0.69 and 99.62 ± 0.54, respectively. The analytical findings demonstrate that the proposed approach could be used to quantify Fluticasone propionate and olopatadine hydrochloride simultaneously in Pharmaceutical formulations.