Environmental amoebae and mycobacterial pathogenesis

Environmental amoebae have been shown to be a host to pathogenic mycobacteria. Mycobacterium avium, Mycobacterium marinum, and Mycobacterium peregrinum can all grow inside Acanthamoeba and other environmental amoebae. Once ingested by Acanthamoeba, M. avium upregulates a number of genes, many of the...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 465; p. 433
Main Authors Harriff, Melanie, Bermudez, Luiz E
Format Journal Article
LanguageEnglish
Published United States 2009
Subjects
Acanthamoeba - microbiology
Amoeba - microbiology
Gene Expression Regulation, Bacterial
Genes, Bacterial
Host-Pathogen Interactions
Humans
Macrophages - microbiology
Mycobacterium - genetics
Mycobacterium - pathogenicity
Mycobacterium avium - genetics
Mycobacterium avium - pathogenicity
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Summary:Environmental amoebae have been shown to be a host to pathogenic mycobacteria. Mycobacterium avium, Mycobacterium marinum, and Mycobacterium peregrinum can all grow inside Acanthamoeba and other environmental amoebae. Once ingested by Acanthamoeba, M. avium upregulates a number of genes, many of them similar to genes upregulated upon phagocytosis of M. avium by macrophages. Mycobacteria ingested by amoebae grow intracellularly, acquiring an invasive phenotype, evident when the bacterium escapes the infected amoeba. Once inside of amoeba, it has been shown that mycobacteria are protected from antibiotics and disinfectants, such as chlorine. This chapter describes methods employed for the study of the interaction of M. avium and Acanthamoeba.
ISSN:1940-6029
DOI:10.1007/978-1-59745-207-6_28