First report of Tomato mottle mosaic virus in Solanum lycopersicum seeds in The Netherlands and intercepted in seed imported from Asia

• Published in: New disease reports Vol. 45; no. 2
• Main Authors: Fowkes, A. R., Botermans, M., Frew, L., Koning, P. P.M, Buxton‐Kirk, A., Westenberg, M., Ward, R., Schenk, M.F., Webster, G., Alraiss, K., Harju, V., Skelton, A., Conyers, C., Barrett, B., Adams, I. P., McGreig, S., Fox, A., Vazquez‐Iglesias, I.
• Format: Journal Article
• Language: English
• Published: Reading John Wiley & Sons, Inc 01.04.2022
• Subjects: Genomes; Plant viruses; Seeds; Solanum lycopersicum; Tobamovirus; ToMMV; Netherlands; United Kingdom > UK; Asia; China
• ISSN: 2044-0588; 2044-0588
• DOI: 10.1002/ndr2.12067

A total of 3,000 seeds were tested for Tomato mottle mosaic virus (ToMMV; genus Tobamovirus) using the RT-PCR test (F-5476 and R-6287) from Levitzky et al. Maximum-likelihood tree (500 bootstraps) of complete Tomato mottle mosaic virus (ToMMV) genome sequences available in GenBank and the sequences obtained from the UK and The Netherlands Separately, in April 2020 and July 2021, three seed samples of 3,000 seeds of S. lycopersicum (36783676 origin Netherlands; 36783860 and 41106813 imported from China) were submitted to the NPPO of The Netherlands for Tomato brown rugose fruit virus (ToBRFV) testing and sequenced by HTS as described in Hammond et al. TABLE 1 Tomato mottle mosaic virus-specific RT-qPCR test developed and validated at Fera Science Ltd Primer/Probe name Sequence (5′-3′) ToMMV 5228-F CGT GGT GGT GTC AGC ATC TG ToMMV 5278-R TGA AGC GAC ACT CGG ATC G ToMMV 5249-Pe [FAM]-TTG GTC GAT AAA AGA ATG CAA AGA GCG G-[TAMRA] ACKNOWLEDGEMENTS UK work was funded under the Defra-Fera Long Term Service Agreement.