Virulence of Spodoptera Litura Nuclear Polyhedrosis Virus (SLNPV) with kaolin as carrier material on spodoptera litura and tetragonula laeviceps on soybean

• Published in: IOP conference series. Earth and environmental science Vol. 672; no. 1; p. 12097
• Main Authors: Erdiansyah, I, Syarief, M, Taufika, R
• Format: Journal Article
• Language: English
• Published: Bristol IOP Publishing 01.03.2021
• Subjects: Contamination; Data analysis; Kaolin; Larvae; Mortality; Plant protection; Soybeans; Virulence
• ISSN: 1755-1307; 1755-1315
• DOI: 10.1088/1755-1315/672/1/012097

Abstract The objective of research is determined the virulence of different concentrations of SlNPV-JTM 97C with kaolin as carrier material on S. litura and T. laeviceps mortality on soybean . The Research was conducted in Plant Protection Laboratory of State polytechnic of Jember, from June to September 2020. The lethal concentration (LC 50 and LC 95 ) SlNPVusing Polo Plus 1.0, with the IRAC version 3, method number 008. The S. litura mortality test used Completely Randomind Design with 5 treatments and 3 replications. The treatments were contamination of larvae diet by SlNPV with control (the third instar larvae without contamination SlNPV), 0,1%, 0,2%, 0,3% and 0,5%. These concentrations are equivalent to the concentrations of 0.1.2x 10 8 PIBs/g, 2.4x 10 8 PIBs/g, 3.6 x 10 8 PIBs/g and 6.0 x 10 8 PIBs/g, respectively. Each test was repeated three times. Mortality percentage of larvae were observed as experiment data. Those data were analyzed by Analysis of Variant and Least Significant Difference (LSD) 5%. SlNPV virulence test against T. laeviceps using a concentration that can cause S. litura mortality ≥ 70%. The results of the research indicated that LC 50 of SlNPV with kaolin as carrier against S. litura 3 rd instar was 0.2%, LC 95 was 0.5%, effective concentration of SlNPV to control S. litura 3 rd instar was 0.3% to 0.5%, SlNPV concentration at 0.3% to 0.5%, non-toxic to T. laeviceps .