Lactococcus lactis and Lactococcus garvieae identified from Aceh buffalo (Bubalus bubalis) associated lactic acid bacterial isolates

• Published in: IOP conference series. Earth and environmental science Vol. 711; no. 1; p. 12005
• Main Authors: Suhartono, S, Misrahanum, M, Yulvizar, C, Khairami, I R
• Format: Journal Article
• Language: English
• Published: Bristol IOP Publishing 01.03.2021
• Subjects: Antiinfectives and antibacterials; Bacteria; Bacteriocins; Biochemistry; Buffalo; E coli; Lactic acid; Lactic acid bacteria; Lactococcus garvieae; Lactococcus lactis; Meat; rRNA 16S
• ISSN: 1755-1307; 1755-1315
• DOI: 10.1088/1755-1315/711/1/012005

Abstract Lactic acid bacteria (LAB) are one of the beneficial bacterial groups due to their metabolic activities, including production of bacteriocin that are potentially applied as biopreservation. The objectives of the study were to identify LAB isolate coded as BK-03 using biochemical and molecular approach as well as to determine the potency of the isolate in producing bacteriocin. The isolate of BK-03 recovered from the meat of Aceh indigenous buffalo was subjected to biochemical and molecular identification. Based on biochemical identification using the API 50 CHL, the LAB isolate of BK-03 was identified as Lactococcus lactis ssp lactis 1 with 98.6% proximity percentage. However, different results were obtained from molecular approach using 16S rRNA, as the isolate was identified as Lactococcus garvieae with a a similarity index of 99%. The results of the antimicrobial assay showed that the crude extracts of BK-03 bacteriocin did not show an inhibition zone to Escherichia coli , but had an inhibitory zone against Staphylococcus aureus with a average diameter of 7.45 mm.