Use of starch-gelled medium for tissue culture of some fruit crops

Six cultivars of apple and two of red raspberry consistently produced equal or significantly better shoot proliferation on modified Murashige and Skoog medium gelled with a mixture of corn starch and Gelrite than on the same medium gelled with agar. Two pear cultivars grown on starch-Gelrite medium...

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Bibliographic Details
Published inPlant cell, tissue and organ culture Vol. 43; no. 3; pp. 207 - 213
Main Authors Zimmerman, R.H, Bhardwaj, S.V, Fordham, I.M
Format Journal Article
LanguageEnglish
Published 01.12.1995
Subjects
agar
benzyladenine
cultivars
culture media
gelling agents
gelrite
gels
genetic variation
growth rate
hyperhydricity
maltose
Malus domestica
Pyrus communis
Rubus idaeus
shoots
starch
tissue culture
vitrification
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Summary:Six cultivars of apple and two of red raspberry consistently produced equal or significantly better shoot proliferation on modified Murashige and Skoog medium gelled with a mixture of corn starch and Gelrite than on the same medium gelled with agar. Two pear cultivars grown on starch-Gelrite medium produced hyperhydric shoots and almost no growth, but the addition of a polysaccharide hydric control ('antivitrifying') agent to the medium eliminated hyperhydricity. The resulting shoot proliferation equaled or exceeded that on the agar-gelled medium. The starch-Gelrite mixture is easy to prepare and gelling agent costs are only 10-15% of agar, or less if starch is purchased in bulk. Although the opaque gray-white medium makes it more difficult to detect internal contaminants, external contaminants are easily discerned.
ISSN:0167-6857
1573-5044
DOI:10.1007/BF00039946