Enhanced survival of Lacticaseibacillus rhamnosus in simulated gastrointestinal conditions using layer-by-layer encapsulation
Objective The release behavior of Lacticaseibacillus rhamnosus from single bilayer microcapsules of alginate-chitosan (AC) and its double bilayer (ACAC) was investigated in simulated gastric fluid (SGF) and simulated intestinal fluid (SIF). Methods Multilayer polyelectrolyte AC microcapsules were fa...
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Published in | Biotechnology letters Vol. 44; no. 11; pp. 1277 - 1286 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Dordrecht
Springer Netherlands
01.11.2022
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Objective
The release behavior of
Lacticaseibacillus rhamnosus
from single bilayer microcapsules of alginate-chitosan (AC) and its double bilayer (ACAC) was investigated in simulated gastric fluid (SGF) and simulated intestinal fluid (SIF).
Methods
Multilayer polyelectrolyte AC microcapsules were fabricated using the layer-by-layer (LbL) self-assembly technique through electrostatic interactions.
Results
AC and ACAC microcapsules kept their integrity and mechanical stability in simulated gastric conditions. Bacterial cells remained inside microcapsules in SGF and dissolution of microcapsules was observed in SIF. To improve the bacterial survivability,
L. rhamnosus
was co-encapsulated in a double bilayer of AC hydrogels with calcium carbonate as an antacid agent.
Conclusions
The LbL self-assembly technology provides stable and target release for ACAC microcapsules. Therefore, the double bilayer polyelectrolyte microcapsules have a remarkable potential for successful application in the targeted and controlled delivery of different probiotics and drugs. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0141-5492 1573-6776 |
DOI: | 10.1007/s10529-022-03289-0 |