A microscopic study of chlorpromazine (largactil) treated axolotl larvae

• Published in: Development (Cambridge) Vol. 12; no. 3; pp. 447 - 456
• Main Authors: EYAL-GILADI, H, EYAL, Z, ESHEL, S
• Format: Journal Article
• Language: English
• Published: England Cambridge University Press for The Company of Biologists Limited 01.09.1964
• Subjects: Abnormalities, Drug-Induced; Ambystoma; Ambystoma mexicanum; Animals; Blood Vessels; Chlorpromazine; Embryo, Mammalian; Embryo, Nonmammalian; Germ Layers; Larva; Nervous System; Pharmacology; Toxicology; Urodela; CHLORPROMAZINE; BLOOD VESSELS; EMBRYO; PHARMACOLOGY; EXPERIMENTAL LAB STUDY; NERVOUS SYSTEM; SALAMANDERS; ABNORMALITIES, DRUG-INDUCED; GERM LAYERS; TOXICOLOGIC REPORT
• ISSN: 0950-1991; 0022-0752; 1477-9129
• DOI: 10.1242/dev.12.3.447

In previous publications (Eyal-Giladi & Eyal, 1962; Eyal & Eyal-Giladi, 1963) some of the macroscopic effects produced by chlorpromazine (CPZ) on the development and growth rate of Axolotl embryos have been reported. They include different abnormalities (microcephaly, defects in eyes, gills and pigmentation) depending on the CPZ concentration used and the length of the immersion period. In addition, larvae subjected to high CPZ concentrations and longer time of treatment proved, at the time of fixation, to be smaller than the controls, whereas larvae treated by low CPZ concentrations tended to grow bigger than the controls. The teratogenic effect and the influence on growth being established, a microscopic examination of the same material was undertaken. Three experimental series were run, each starting with a different initial developmental stage (Harrison's stages 10–11, 15–16 and 29–30 respectively). Specia's (Paris) CPZ chlorhydrate powder was dissolved in sterile tap water.