Interaction of nanoplastics with simulated biological fluids and their effect on the biofilm formation

• Published in: Environmental science and pollution research international Vol. 29; no. 53; pp. 80775 - 80786
• Main Authors: Saygin, Hasan, Baysal, Asli
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.11.2022; Springer Nature B.V
• Subjects: Aquatic Pollution; Atmospheric Protection/Air Quality Control/Air Pollution; Biofilms; Chemical composition; Controlled conditions; Earth and Environmental Science; Ecotoxicology; Environment; Environmental Chemistry; Environmental Health; Environmental science; Fluids; Functional groups; Hydroxyl groups; Plastic debris; Plastic pollution; Research Article; Saliva; Simulation; Surface properties; Waste Water Technology; Water Management; Water Pollution Control; Human; Biofilm; Bacteria; Secondary plastics; Nanoplastics
• ISSN: 0944-1344; 1614-7499; 1614-7499
• DOI: 10.1007/s11356-022-21468-4

Over the last decade, it has become clear that the pollution by plastic debris presents global societal, environmental, and human health challenges. Moreover, humans are exposed to plastic particles in daily life and very limited information is available concerning human health, especially interactions with biological fluids. Therefore, the aim of this study is to investigate the interaction of plastic particles with simulated biological fluids (e.g., artificial saliva, artificial lysosomal fluid, phagolysosomal simulant fluid, and Gamble’s solution) using various exposure stages (2 h to 80 h) and the effect of plastic particles on the formation of Staphylococcus aureus biofilms under simulated biological conditions. The plastic particles incubating various simulated biological fluids were characterized using surface functional groups, zeta potentials, and elemental composition. The results indicated that functional group indices (C–O, C = O, C–H, C = C, C–N, S = O, and OH) decreased compared to the control group during the incubation periods, except for the hydroxyl group index. The FTIR results showed that the hydroxyl group formed with the artificial lysosomal fluid, the phagolysosomal simulant fluid, and Gamble’s solution. With the impact of the declining functional groups, the zeta potentials were more negative than in the control. Moreover, EDX results showed the release of the components in the particles with the interaction of simulated biological fluids as well as new components like P and Ca introduced to the particles. The biofilms were formed in the presence of nanoplastic particles under both controlled conditions and simulated biological conditions. The amount of biofilm formation was mainly affected by the surface characteristics under simulated biological conditions. In addition, the biofilm characteristics were influenced by the O/C and N/C ratios of the plastic particles with the impact of simulated biological fluids.