Detection of antibody to mycoplasma F38 in goat sera by an enzyme-linked immunosorbent assay

An indirect enzyme-linked immunosorbent assay (ELISA) was developed to screen goat sera at a single dilution for antibody to mycoplasma F38. Antibody was detected in sera of six convalescent goats following experimental infection. Antibody was also detected in 34 sera three to four weeks after vacci...

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Bibliographic Details
Published inTropical animal health and production Vol. 21; no. 1; p. 43
Main Authors Wamwayi, H M, Wafula, J S, Litamoi, J K, Nandokha, E N
Format Journal Article
LanguageEnglish
Published United States 01.02.1989
Subjects
Animals
Antibodies, Bacterial - analysis
Bacterial Vaccines - immunology
Enzyme-Linked Immunosorbent Assay - veterinary
Goats - immunology
Immunoglobulin G - analysis
Mycoplasma - immunology
Pleuropneumonia, Contagious - diagnosis
Pleuropneumonia, Contagious - immunology
Vaccination - veterinary
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Summary:An indirect enzyme-linked immunosorbent assay (ELISA) was developed to screen goat sera at a single dilution for antibody to mycoplasma F38. Antibody was detected in sera of six convalescent goats following experimental infection. Antibody was also detected in 34 sera three to four weeks after vaccination. No antibody was detected in 164 sera from goats without a history of vaccination or infection with contagious caprine pleuropneumonia. The ELISA was more sensitive than the complement fixation test in detecting antibody in vaccinated goats.
ISSN:0049-4747
DOI:10.1007/BF02297342