Insulin enhances angiotensin II induced DNA synthesis in vascular smooth muscle cells of the rat

Hypertension has a high prevalence among subjects with decreased insulin sensitivity and/or hyperinsulinemia. Furthermore, angiotensin II plays a pivotal role in the regulation of vascular tone and is known to induce hypertrophy and/or hyperplasia in vascular smooth muscle cells. In the present stud...

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Bibliographic Details
Published inThe Clinical investigator Vol. 71; no. 5; p. 379
Main Authors Ko, Y, Sachinidis, A, Wieczorek, A J, Appenheimer, M, Düsing, R, Vetter, H
Format Journal Article
LanguageEnglish
Published Germany 01.05.1993
Subjects
Angiotensin II - pharmacology
Animals
DNA - biosynthesis
Dose-Response Relationship, Drug
Drug Synergism
Female
Insulin - pharmacology
Muscle, Smooth, Vascular - drug effects
Rats
Rats, Inbred WKY
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Summary:Hypertension has a high prevalence among subjects with decreased insulin sensitivity and/or hyperinsulinemia. Furthermore, angiotensin II plays a pivotal role in the regulation of vascular tone and is known to induce hypertrophy and/or hyperplasia in vascular smooth muscle cells. In the present study, the effect of insulin on angiotensin II induced smooth muscle cell growth (Wistar-Kyoto rat) was investigated. Cell growth was assessed by the measurement of [3H]thymidine incorporation into cell DNA. Insulin in a concentration range of 1.7 x 10(-10)-1.7 x 10(-6) M lacked any effect on cell DNA synthesis. However, insulin enhanced the angiotensin II induced DNA synthesis in a concentration-dependent manner. This effect was similar in cells with a weak and in cells with a marked response in DNA synthesis to stimulation with 100 nM angiotensin II. In conclusion, insulin is able to enhance angiotensin II induced DNA synthesis and may therefore function as a growth cofactor in vascular smooth muscle cells.
ISSN:0941-0198
DOI:10.1007/BF00186627