Selection of HILIC columns to handle matrix effect due to phospholipids

Phospholipids from biological samples are a source of matrix effect in liquid chromatography mass spectrometry. While the behavior of phospholipids has been documented under reversed-phase chromatography, there is a lack of information concerning the selectivity of hydrophilic interaction chromatogr...

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Bibliographic Details
Published inBioanalysis Vol. 1; no. 1; p. 57
Main Authors Mess, Jean-Nicholas, Côté, Cynthia, Bergeron, Annik, Furtado, Milton, Garofolo, Fabio
Format Journal Article
LanguageEnglish
Published England 01.04.2009
Subjects
Antihypertensive Agents - blood
Chromatography, Liquid
Chromatography, Reverse-Phase
Humans
Hydrophobic and Hydrophilic Interactions
Phospholipids - blood
Phospholipids - chemistry
Reserpine - blood
Solid Phase Extraction
Tandem Mass Spectrometry
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Summary:Phospholipids from biological samples are a source of matrix effect in liquid chromatography mass spectrometry. While the behavior of phospholipids has been documented under reversed-phase chromatography, there is a lack of information concerning the selectivity of hydrophilic interaction chromatography (HILIC) towards phospholipids. Human plasma extracts were used to evaluate retention times and matrix effects associated with phospholipids under HILIC conditions. It was observed that phosphatidylcholine and lysophosphatidylcholine phospholipid retention times vary greatly between columns operated in different HILIC conditions. Therefore, matrix effects associated with phospholipids could present a quantitation problem if not evaluated thoroughly during method development. Analytical chemists should carefully choose the right combination of sample preparation and chromatographic conditions when working under HILIC conditions to avoid variable results.
ISSN:1757-6199
DOI:10.4155/bio.09.9