Mapping of the focal adhesion kinase (Fadk) gene to mouse chromosome 15 and human chromosome 8

• Published in: Mammalian genome Vol. 6; no. 2; p. 123
• Main Authors: Fiedorek, Jr, F T, Kay, E S
• Format: Journal Article
• Language: English
• Published: United States 01.02.1995
• Subjects: Animals; Base Sequence; Cell Adhesion Molecules - genetics; Chromosome Mapping; Chromosomes, Human, Pair 8; Crosses, Genetic; Focal Adhesion Kinase 1; Focal Adhesion Protein-Tyrosine Kinases; Genes; Humans; Mice - genetics; Mice, Inbred C57BL; Molecular Sequence Data; Muridae - genetics; Polymerase Chain Reaction; Protein-Tyrosine Kinases - genetics
• ISSN: 0938-8990
• DOI: 10.1007/BF00303256

Focal adhesion kinase (pp125FAK or FAK) is a cytoplasmic protein-tyrosine kinase stimulated in response to cell interactions with extracellular matrix components and by exposure to a variety of agonists, including neuropeptides. FAK lacks Src-homology SH2 and SH3 domains, is highly conserved across species, and may represent the prototype for a tyrosine kinase family involved in novel signal transduction pathways. We have identified sequence variants in the 3' untranslated regions of the focal adhesion kinase gene in mice and used a PCR-based oligonucleotide hybridization assay to map the mouse gene (Fadk) to Chromosome (Chr) 15 distal to the myelocytomatosis protooncogene (Myc). The human homolog (PTK2) has been assigned to human Chr 8 on a panel of somatic hybrid cell lines. On the basis of synteny of mouse and human chromosomal maps, the position of the human PTK2 gene probably corresponds to human Chr 8q24-qter.