Design of Gd3+-immobilized two-dimensional magnetic magadiite nanosheets for highly selective enrichment of phosphopeptides

• Published in: Mikrochimica acta (1966) Vol. 188; no. 10; p. 327
• Main Authors: Jiang, Dandan, Lv, Siqi, Han, Xue, Duan, Limei, Liu, Jinghai
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01.10.2021; Springer Nature B.V
• Subjects: Adsorbents; Analytical Chemistry; Casein; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Enrichment; Gadolinium; Ions; Iron oxides; Magnetic properties; Mass spectrometry; Microengineering; Morphology; Nanochemistry; Nanocomposites; Nanosheets; Nanotechnology; Original Paper; Serum albumin; Magnetic nanoparticle; MALDI TOF-MS; Immobilized metal affinity chromatography; Protein phosphorylation
• ISSN: 0026-3672; 1436-5073
• DOI: 10.1007/s00604-021-04972-1

Exfoliated magadiite nanosheets embedded with Fe 3 O 4 were constructed. Advantage was taken of the strong coordination between the silanol groups in magadiite nanosheets and the Gd 3+ ion to prepare the final adsorbent, Gd 3+ -immobilized magnetic magadiite nanosheets. The adsorbent with two-dimensional (2D) morphology offered high surface area and abundant Gd 3+ contents for phosphopeptides enrichment, on which Fe 3 O 4 with positive electricity incorporated the magnetic properties. Combining with matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI TOF-MS), the method showed low detection limit (0.05 fmol). The feasibility of using the 2D nanocomposite for phosphopeptides enrichment was demonstrated using mixtures of β -casein and bovine serum albumin (1:5000). The standard deviation of captured phosphopeptides in three repeated experiments were in the range 0.15–0.42 (< 0.5% RSD). Further evaluation revealed that the nanocomposite was capable of enriching phosphopeptides from non-fat milk, human saliva, and serum. Graphical abstract A novel Gd 3+ -immobilized two-dimensional magnetic magadiite nanosheets-based enrichment platform was designed. The developed material was employed as the adsorbent for the selective enrichment of phosphopeptides by coupling with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The material was successfully applied to enrich phosphopetides from standard peptide mixtures, nonfat milk, human saliva, and serum.