Design of Gd3+-immobilized two-dimensional magnetic magadiite nanosheets for highly selective enrichment of phosphopeptides
Exfoliated magadiite nanosheets embedded with Fe 3 O 4 were constructed. Advantage was taken of the strong coordination between the silanol groups in magadiite nanosheets and the Gd 3+ ion to prepare the final adsorbent, Gd 3+ -immobilized magnetic magadiite nanosheets. The adsorbent with two-dimens...
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Published in | Mikrochimica acta (1966) Vol. 188; no. 10; p. 327 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Vienna
Springer Vienna
01.10.2021
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Exfoliated magadiite nanosheets embedded with Fe
3
O
4
were constructed. Advantage was taken of the strong coordination between the silanol groups in magadiite nanosheets and the Gd
3+
ion to prepare the final adsorbent, Gd
3+
-immobilized magnetic magadiite nanosheets. The adsorbent with two-dimensional (2D) morphology offered high surface area and abundant Gd
3+
contents for phosphopeptides enrichment, on which Fe
3
O
4
with positive electricity incorporated the magnetic properties. Combining with matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI TOF-MS), the method showed low detection limit (0.05 fmol). The feasibility of using the 2D nanocomposite for phosphopeptides enrichment was demonstrated using mixtures of
β
-casein and bovine serum albumin (1:5000). The standard deviation of captured phosphopeptides in three repeated experiments were in the range 0.15–0.42 (< 0.5% RSD). Further evaluation revealed that the nanocomposite was capable of enriching phosphopeptides from non-fat milk, human saliva, and serum.
Graphical abstract
A novel Gd
3+
-immobilized two-dimensional magnetic magadiite nanosheets-based enrichment platform was designed. The developed material was employed as the adsorbent for the selective enrichment of phosphopeptides by coupling with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The material was successfully applied to enrich phosphopetides from standard peptide mixtures, nonfat milk, human saliva, and serum. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0026-3672 1436-5073 |
DOI: | 10.1007/s00604-021-04972-1 |