A simple and effective scaffold for mouse hepatic stellate cell primary culture

• Published in: American Journal of Physiology: Cell Physiology Vol. 324; no. 6; pp. C1213 - C1222
• Main Authors: Le, Trinh Van, Diep, Thai Trieu Ngoc, Nhan, Vo Thanh, Uyen, Nguyen Luu Thao, Thanh, Dang Minh, Truong, Nhung Hai
• Format: Journal Article
• Language: English
• Published: United States American Physiological Society 01.06.2023
• Subjects: Actin; Animals; Cell activation; Cell adhesion molecules; Cell culture; Cells, Cultured; Collagen (type I); Collagen Type I - metabolism; Cytology; E-cadherin; Fibrin; Hepatic Stellate Cells - metabolism; Liver; Liver Cirrhosis - metabolism; Mice; Morphology; N-Cadherin; Physical characteristics; Plastics; Smooth muscle; Stellate cells; hepatic stellate cells; fibrosis; morphology; scaffold; extracellular matrix
• ISSN: 0363-6143; 1522-1563
• DOI: 10.1152/ajpcell.00001.2023

Scaffold-based culture is necessary for hepatic stellate cells (HSCs) because HSCs are promptly autoactivated under plastic conditions. Our research aims to investigate the potential and role of fibrin scaffold in reducing autoactivation, maintaining cell function, and extending the in vitro culture time of primary HSCs. HSCs were isolated from BALB/c mice and cultured on the surface of plastic, Matrigel, and fibrin gel. HSC's characteristics, including recovery, morphology, proliferation, lipid droplet (LD) storage, and activation were evaluated. Cell recovery was 86%, 80%, and 60% in fibrin, Matrigel, and plastic, respectively ( < 0.05). HSCs cultured on a plastic dish were autoactivated until with high proliferation, loss of cytoplasmic LD lipid droplets, and increased expression of activation markers, including alpha-smooth muscle actin (α-sma) and collagen type I. In contrast, these phenomena were reduced in Matrigel and fibrin-based cultures ( < 0.05). HSC culture in fibrin scaffold was associated with altered expression of cell adhesion molecules, including increased and inhibited . HSCs were more stellate-like in morphology in fibrin than in the Matrigel scaffold. Interestingly, fibrin-scaffold-embedded culture was able to maintain HSC quiescent state for up to 14 days in vitro. Fibrin gel could provide a potential scaffold for primary HSC culture while preserving cell function and extending primary HSC in vitro culture time. Fibrin gel is appropriate for maintaining quiescence characteristics in primary culture of mouse hepatic stellate cells. Embedded culture of hepatic stellate cells in fibrin gel simulates in vivo cell morphology. Stiffness and adhesion molecules of fibrin gel play a crucial role in the hepatic stellate cell's primary culture.