Cystathionase: a potential cytoplasmic marker of hematopoietic differentiation

Cysteine auxotrophy and absence of cystathionase (CSE) has been associated with certain human and rodent leukemic cell lines. To determine whether this state was a marker of malignant transformation or of cellular differentiation, CSE content was measured in 16 well characterized human leukemia-lymp...

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Bibliographic Details
Published inBlut Vol. 47; no. 1; p. 31
Main Authors Link, D, Drebing, C, Glode, L M
Format Journal Article
LanguageEnglish
Published Germany 01.07.1983
Subjects
Bone Marrow Cells
Cell Differentiation
Cell Line
Colony-Forming Units Assay
Cystathionine - metabolism
Cystathionine gamma-Lyase - metabolism
Cysteine - metabolism
Cytoplasm - enzymology
Hematopoietic Stem Cells - enzymology
Humans
Leukemia - enzymology
Lyases - metabolism
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Summary:Cysteine auxotrophy and absence of cystathionase (CSE) has been associated with certain human and rodent leukemic cell lines. To determine whether this state was a marker of malignant transformation or of cellular differentiation, CSE content was measured in 16 well characterized human leukemia-lymphoma cell lines. Enzyme was easily detected in several lines but its level did not correlate with a proposed scheme of differentiation based on cell-surface markers. However, the apparent absence of enzyme in human bone marrow CFU-C determined by growth experiments suggests reduced levels of CSE may be a marker of cytoplasmic immaturity.
ISSN:0006-5242
DOI:10.1007/BF00321048