A new method for intratubular blockade in micropuncture experiments

Criteria for a reliable blockade of tubular flow are generally discussed and experimental evidence is presented that solid paraffin meets all the necessary criteria in rat kidney tubules when an immobile and tight sealing intratubular blockade is requested. A special microinjection apparatus for the...

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Bibliographic Details
Published inPflügers Archiv Vol. 354; no. 2; p. 197
Main Authors Gutsche, H U, Müller-Surr, R, Hegel, U, Hierholzer, K, Lüderitz, S
Format Journal Article
LanguageEnglish
Published Germany 01.01.1975
Subjects
Animals
Gels
Kidney Tubules - physiology
Microinjections - instrumentation
Nephrons - physiology
Paraffin
Punctures - methods
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Summary:Criteria for a reliable blockade of tubular flow are generally discussed and experimental evidence is presented that solid paraffin meets all the necessary criteria in rat kidney tubules when an immobile and tight sealing intratubular blockade is requested. A special microinjection apparatus for the application of paraffin is described. Furthermore, several alternative methods and materials have been tested and have been found to be inferior to solid paraffin.
ISSN:0031-6768
DOI:10.1007/BF00579949