Insecticide application did not reveal any impact of herbivory on plant roots in boreal forests

• Published in: Applied soil ecology : a section of Agriculture, ecosystems & environment Vol. 178; p. 104554
• Main Authors: Kozlov, Mikhail V., Zverev, Vitali
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.10.2022
• Subjects: Background root herbivory; Boreal forest; Insecticide; Research methodology; Boreal forest; Background root herbivory; Insecticide; Research methodology
• ISSN: 0929-1393; 1873-0272
• DOI: 10.1016/j.apsoil.2022.104554

The levels of belowground herbivory in natural ecosystems remain practically undetermined, and nothing is known regarding the geographic and/or climatic variations in belowground herbivory. We endeavoured to narrow this knowledge gap by exploring the latitudinal changes in the intensity of background root herbivory in boreal forest ecosystems by conducting a herbivore exclusion experiment in 10 forested sites from 60°N to 69°N in northwestern Russia. We found no statistically significant differences in fine root biomass between diazinon-treated and control plots, nor did the differences show any latitudinal change. From biomass of root-feeding macrofauna we estimated that root herbivory in our sites averages 0.57 %. This low level of root herbivory could not be quantified reliably by herbivore exclusion experiments; therefore, we suggest that macroecological patterns in root herbivory are invoked from simultaneous measurements of the biomasses of fine roots and of root-feeders. More data on the efficiency of conversion of the food ingested by root-feeding invertebrates is needed to increase the accuracy of the suggested method of estimation of root herbivory. •We treated soil with insecticide to quantify root herbivory in boreal forests.•Fine root biomass did not differ between insecticide-treated and control plots.•Biomass of root-feeding insects suggests that root herbivory ranges from 0.1 to 0.3 %.•This low level of root herbivory could not be quantified by the method used.