Zoogloea dura sp. nov., a N2-fixing bacterium isolated from forest soil and emendation of the genus Zoogloea and the species Zoogloea oryzae and Zoogloea ramigera

• Published in: International Journal of Systematic and Evolutionary Microbiology Vol. 70; no. 10; pp. 5312 - 5318
• Main Authors: Dahal, Ram Hari, Chaudhary, Dhiraj Kumar, Kim, Dong-Uk, Kim, Jaisoo
• Format: Journal Article
• Language: English; Japanese
• Published: Microbiology Society 01.10.2020
• ISSN: 1466-5026; 1466-5034; 1466-5034
• DOI: 10.1099/ijsem.0.004416

A motile, Gram-stain-negative, rod-shaped bacterium, designated G-4-1-14 T , was obtained from forest soil sampled at Gwanggyo mountain, Gyeonggi-do, Republic of Korea. Cells were colourless, aerobic, grew optimally at 28–35 °C and hydrolysed DNA and casein. Phylogenetic analysis based on its 16S rRNA gene sequence revealed that strain G-4-1-14 T formed a lineage within the genus Zoogloea . The closest members were Zoogloea resiniphila ATCC 70068 T (98.6 % sequence similarity), Zoogloea caeni EMB43 T (98.2 %), Zoogloea oryzae A-7 T (97.7 %), Zoogloea ramigera IAM 12136 T (96.9 %) and Zoogloea oleivorans Buc T (96.2 %). The major respiratory quinone was ubiquinone-8 and the principal polar lipids were phosphatidylethanolamine, phosphatidyl- N -methylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The predominant cellular fatty acids were summed feature 3 (iso-C 15 :0 2-OH/C 16  : 1 ω 7 c ) and C 16 : 0 . The DNA G+C content was 65.9 mol%. The average nucleotide identity and digital DNA–DNA hybridization relatedness values between strain G-4-1-14 T and other type strains were ≤81.6 and ≤24.9 %, respectively, which are below the species demarcation thresholds. Based on the results of phenotypic, phylogenetic and genomic analyses, strain G-4-1-14 T represents a novel species in the genus Zoogloea , for which the name Zoogloea dura sp. nov. is proposed. The type strain is G-4-1-14 T (=KACC 21618 T =NBRC 114358 T ). In addition, we propose emendation of the genus Zoogloea and the species Zoogloea oryzae and Zoogloea ramigera .