Characterization of Alu family repetitive sequences which flank human beta-type globin genes

Heteroduplex mapping has determined the size, location, and orientation of three Alu family sequences from the human beta-type globin gene cluster. Two of these sequences have the same orientation. One (231 bp long) is 2 kb to the 5' side of the B gamma-globin gene and the other (222 bp) is l k...

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Bibliographic Details
Published inBioscience reports Vol. 1; no. 4; p. 309
Main Authors Coggins, L W, Lanyon, W G, Slater, A A, Grindlay, G J, Paul, J
Format Journal Article
LanguageEnglish
Published England 01.04.1981
Subjects
Base Sequence
DNA
DNA Restriction Enzymes
DNA, Recombinant
Globins - genetics
Humans
Nucleic Acid Heteroduplexes
Nucleic Acid Hybridization
Repetitive Sequences, Nucleic Acid
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Summary:Heteroduplex mapping has determined the size, location, and orientation of three Alu family sequences from the human beta-type globin gene cluster. Two of these sequences have the same orientation. One (231 bp long) is 2 kb to the 5' side of the B gamma-globin gene and the other (222 bp) is l kb 5' to the pseudo-beta-l-globin gene. The third (300 bp), 3-4 kb 3' to the pseudo-beta-l-globin gene, has the opposite orientation. Their orientations relative to five previously characterized Alu sequences from this cluster have been established. One of these, 2.5 kb 5' to the epsilon-globin gene, was shown by Southern blot hybridization to be similar but not identical to other family members, whereas the region separating it from a neighbouring inverted repeat is not widely distributed in the human genome.
ISSN:0144-8463
DOI:10.1007/BF01114870