Evidence for the presence of a cell-surface ribonuclease in mechanically prepared rat-liver cells in suspension

Rat-liver parenchymal cells obtained in suspension by a mechanical method are shown to contain a cell-surface nuclease(s) that rapidly degrades exogenously added total Escherichia coli RNA. However, no acid-soluble products are formed; all the degradation products in the incubation medium sediment i...

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Bibliographic Details
Published inBioscience reports Vol. 2; no. 10; p. 751
Main Authors Sirdeshmukh, R, Bhargava, P M
Format Journal Article
LanguageEnglish
Published England 01.10.1982
Subjects
Animals
Cell Membrane - enzymology
Centrifugation, Density Gradient
Female
Kinetics
Liver - enzymology
Male
Molecular Weight
Rats
Rats, Inbred Strains
Ribonucleases - isolation & purification
Ribonucleases - metabolism
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Summary:Rat-liver parenchymal cells obtained in suspension by a mechanical method are shown to contain a cell-surface nuclease(s) that rapidly degrades exogenously added total Escherichia coli RNA. However, no acid-soluble products are formed; all the degradation products in the incubation medium sediment in the 4-5S RNA region on a sucrose density gradient. A part of the degraded RNA seems to be taken up by the cells; the uptake of the degradation products, presumably derived from rRNAs, is more than that of purified 4-5S RNA. Most of the RNA taken up by the cell sediments in the 4-5S region; only a small proportion is degraded to acid-soluble material within the cell.
ISSN:0144-8463
DOI:10.1007/BF01114934