Oligomeric Proanthocyanidin Complex from Avocado Seed as A Promising α-glucosidase Inhibitor: Characteristics and Mechanisms
Although considered an abundant source of agricultural by-products, avocado ( Mill.) seed, with several biological activities and bioactive components, might become a promising resource for phytopharmaceutical development. In this study, through bioassay-guided isolation of the main -glucosidase inh...
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Published in | Planta medica Vol. 89; no. 3; p. 316 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Germany
01.03.2023
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Subjects | |
Online Access | Get more information |
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Summary: | Although considered an abundant source of agricultural by-products, avocado (
Mill.) seed, with several biological activities and bioactive components, might become a promising resource for phytopharmaceutical development. In this study, through bioassay-guided isolation of the main
-glucosidase inhibitors in avocado seed, we discovered the major
-glucosidase inhibitor to be avocado seed oligomeric proanthocyanidin complex (ASOPC). Thiolysis and UPLC-DAD-HRESIMS showed the presence of A- and B-type procyanidins, and B-type propelargonidin with (epi)afzelechin as extension unit. Mean degree of polymerization (mDP) of ASOPC was calculated as 7.3 ± 1. Furthermore, ASOPC appeared to be a strong, reversible, competitive inhibitor of
-glucosidase, with IC
value of 0.1 µg/mL, which was significantly lower than Acarbose (IC
= 75.6 µg/mL), indicated that ASOPC is a potential natural
-glucosidase inhibitor. These findings would contribute to the direction of utilizing avocado seed bioactive components with the possibility to be used as natural anti-diabetic agents. |
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ISSN: | 1439-0221 |
DOI: | 10.1055/a-1878-3916 |