An antibody-lectin sandwich assay for the determination of CA125 antigen in ovarian cancer patients

A two-step forward sandwich assay was developed for the determination of the ovarian tumour associated glycoconjugate antigen CA125 with anti-CA125 Monoclonal antibody B27.1 on the solid phase and 125I-labelled wheat germ lectin as tracer in the solution phase. This Mab-lectin heterosandwich assay w...

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Bibliographic Details
Published inGlycoconjugate journal Vol. 13; no. 4; pp. 513 - 517
Main Authors Madiyalakan, R, Kuzma, M, Noujaim, A A, Suresh, M R
Format Journal Article
LanguageEnglish
Published United States 01.08.1996
Subjects
Antibodies - immunology
Antibodies, Monoclonal
Antigen-Antibody Reactions
CA-125 Antigen - blood
Female
Humans
Immunoassay - methods
Lectins - immunology
Ovarian Neoplasms - immunology
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Summary:A two-step forward sandwich assay was developed for the determination of the ovarian tumour associated glycoconjugate antigen CA125 with anti-CA125 Monoclonal antibody B27.1 on the solid phase and 125I-labelled wheat germ lectin as tracer in the solution phase. This Mab-lectin heterosandwich assay was optimized and the clinical utility was evaluated in sera from healthy volunteers and ovarian cancer patients. A correlation was established between Mab-lectin assay and the dual monoclonal antibody sandwich assay, TRUQUANTOV2 RIA, that uses the same MAb B27.1 on the solid phase and a second 125I-labelled B43.13 MAb in the solution phase. A potentially improved clinical utility is suggested for the Mab-lectin assay. The unique format seems to identify novel isoforms of CA125 with different carbohydrate side chains that would otherwise be undetectable in the MAb-MAb sandwich assay wherein the paratopes are likely directed to protein determinants.
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ISSN:0282-0080
1573-4986
DOI:10.1007/BF00731437