LUCIFERASES FOR THE STUDY OF PROTEIN–PROTEIN INTERACTIONS IN LIVE CELLS AND ANIMALS

Many imaging technologies based on luminescent proteins have proven useful for detecting protein–protein interactions, tracking cells in mice, and monitoring transcriptional regulation of specific genes. Especially, novel bioluminescent proteins have advanced the study of induced protein interaction...

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Bibliographic Details
Published inNano LIFE Vol. 1; no. 1n02; pp. 79 - 87
Main Authors KAFI, A. K. M., HATTORI, MITSURU, OZAWA, TAKEAKI
Format Journal Article
LanguageEnglish
Published World Scientific Publishing Company 01.03.2010
Subjects
luciferase
bioluminescence
complementation
Protein–protein interaction
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Summary:Many imaging technologies based on luminescent proteins have proven useful for detecting protein–protein interactions, tracking cells in mice, and monitoring transcriptional regulation of specific genes. Especially, novel bioluminescent proteins have advanced the study of induced protein interactions and protein modification in live cells and animals. This review focuses on recent developments of bioluminescent probes for quantitative evaluation of specific protein–protein interactions and their spatio-temporal imaging by means of split luciferase complementation techniques. From the comparison between fluorescent and bioluminescent proteins, advantages and drawbacks of the bioluminescence techniques are described.
ISSN:1793-9844
1793-9852
DOI:10.1142/S1793984410000079