Biological and biochemical characterization of a red-eye mutant in Nilaparvata lugens （Hemiptera： Delphacidae）
A red‐eye colony was established in our laboratory in brown planthopper (BPH), Nilaparvata lugens (Stål), a major rice pest in Asia. Except for the red‐eye phenotype, no other differences were observed between the wild‐type (brown eye) and the mutant‐type (red eye) in external characters. Genetic an...
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| Published in | Insect science Vol. 21; no. 4; pp. 469 - 476 |
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| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Beijing
Blackwell Pub
01.08.2014
Blackwell Publishing Ltd Wiley Subscription Services, Inc |
| Subjects | |
| Online Access | Get full text |
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| Summary: | A red‐eye colony was established in our laboratory in brown planthopper (BPH), Nilaparvata lugens (Stål), a major rice pest in Asia. Except for the red‐eye phenotype, no other differences were observed between the wild‐type (brown eye) and the mutant‐type (red eye) in external characters. Genetic analysis revealed that the red‐eye phenotype was controlled by a single autosomal recessive allele. Biological studies found that egg production and egg viability in the red‐eye mutant colony were not significantly different from those in the wild‐type BPH. Biochemical analysis and electronic microscopy examination revealed that the red‐eye mutants contained decreased levels of both xanthommatin (brown) and pteridine (red) and reduced number of pigment granules. Thus, the changes of amount and ratio of the two pigments is the biochemical basis of this red‐eye mutation. Our results indicate that the red‐eye mutant gene (red) might be involved in one common gene locus shared by the two pigments in pigment transportation, pigment granule formation or some other processes. |
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| Bibliography: | A red-eye colony was established in our laboratory in brown planthopper （BPH）, Nilaparvata lugens （Stal）, a major rice pest in Asia. Except for the red-eye phenotype, no other differences were observed between the wild-type （brown eye） and the mutant-type （red eye） in external characters. Genetic analysis revealed that the red-eye phenotype was controlled by a single autosomal recessive allele. Biological studies found that egg produc- tion and egg viability in the red-eye mutant colony were not significantly different from those in the wild-type BPH. Biochemical analysis and electronic microscopy examination revealed that the red-eye mutants contained decreased levels of both xanthommatin （brown） and pteridine （red） and reduced number of pigment granules. Thus, the changes of amount and ratio of the two pigments is the biochemical basis of this red-eye mutation. Our results indicate that the red-eye mutant gene （red） might be involved in one common gene locus shared by the two pigments in pigment transportation, pigment granule formation or some other processes. 11-3386/Q biological characterization, eye pigment, genetic analysis, Nilaparvatalugens, pigment granule, red-eye mutant http://dx.doi.org/10.1111/1744-7917.12049 ark:/67375/WNG-R0ZJQWTQ-T National Basic Research Program of China - No. 2010CB126206 istex:F5FBAA6A7E651CFD7F432EA9C99314D106D62EBD Zhejiang Provincial Natural Science Foundation of China - No. LY12C14002 ArticleID:INS12049 National Natural Science Foundation of China - No. 31201511 The first two authors contributed equally to this work. ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| ISSN: | 1672-9609 1744-7917 |
| DOI: | 10.1111/1744-7917.12049 |