Toxicological analysis and efficacy of 2-phenylchromone on mycobacteria viability and inflammatory response induced by Mycobacterium bovis

• Published in: Phytomedicine Plus : International journal of phytotherapy and phytopharmacology Vol. 1; no. 4; p. 100117
• Main Authors: Moreira, Flora Martinez Figueira, Radai, Joyce Alencar Santos, de Souza, Vanessa Vilamaior, Berno, Claudia Rodrigues, de Araújo, Flavio Henrique Souza, Andrade-Silva, Magaiver, Oliveira, Rodrigo Juliano, Arena, Arielle Cristina, Henriques, Maria das Graças Müller de Oliveira, Kassuya, Candida Aparecida Leite, Croda, Julio
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.11.2021; Elsevier
• Subjects: Acute toxicity; Comet assay; Micronucleus test; Minimum inhibitory concentration; Mycobacterium tuberculosis; Pleurisy; PBS; Micronucleus test; DMSO; MIC; BCG; TNF; Comet assay; OECD; Mycobacterium tuberculosis; ANOVA; Minimum inhibitory concentration; Pleurisy; Acute toxicity
• ISSN: 2667-0313; 2667-0313
• DOI: 10.1016/j.phyplu.2021.100117

Studies have reported that compounds similar to flavone present antimicrobial and anti-inflammatory properties; however these effects are poorly described in the context of mycobacterium infection. Purpose: This study investigated the efficacy of 2-phenylchromone (Flavone) on mycobacteria viability and inflammatory response induced by Mycobacterium bovis, as well as its toxicological potential in in vitro and in vivo models. Methods: In vitro antimycobacterial activity was performed using the resazurin microtiter assay method. In order to investigate the anti-inflammatory action, C57Bl/6 mice were pretreated orally with 2-phenylchromone (1–100 mg/kg) 1 h before the pleurisy induction. The leukocyte migration and cytokine production were evaluated. Acute oral toxicity test, genotoxicity evaluations and splenic phagocytosis assay were also evaluated. The minimum inhibitory concentration of 2-phenylchromone in the presence of the Mycobacterium tuberculosis strain was 28.90 µg/mL. In the BCG-induced pleurisy model, the oral treatment with 2-phenylchromone (1, 10, or 100 mg/kg) caused a significant reduction in the tumor necrosis factor (TNF) levels in the pleural exudate, as well as in the total and differential leukocyte counts. The oral treatment with 2-phenylchromone also did not present acute toxicity or genotoxicity in comet assay and micronucleus tests. However, 2-phenylchromone induced an increase of 1.48-times in splenic phagocytosis in the animals. Our results showed that 2-phenylchromone did not induced toxicity signs and is effective against Mycobacterium viability and reduced the inflammatory response elicited by mycobacteria. [Display omitted]