Construction of an infectious cDNA clone of the apple stem grooving capillovirus (isolate Li-23) genome containing a cauliflower mosaic virus 35S RNA promoter

• Published in: Nippon shokubutsu byōri gakkai Vol. 63; no. 6
• Main Authors: Terauchi, H. (Iwate Univ., Morioka (Japan). Faculty of Agriculture), Magome, H, Yoshikawa, N, Takahashi, T, Inouye, N
• Format: Journal Article
• Language: English
• Published: 01.12.1997
• Subjects: ADN; DNA; NUCLEOTIDE SEQUENCE; PATHOGENICITY; PATOGENICIDAD; PLANT VIRUSES; POUVOIR PATHOGENE; SECUENCIA NUCLEOTIDICA; SEQUENCE NUCLEOTIDIQUE; VIRUS DE LAS PLANTAS; VIRUS DES VEGETAUX
• ISSN: 0031-9473
• DOI: 10.3186/jjphytopath.63.432

The complete nucleotide sequence of the genome of apple stem grooving capillovirus (ASGV) isolate Li-23 from lily was determined. The isolate Li-23 genome consists of 6496 nucleotides excluding poly(A) tail and contains two overlapping open reading frames (ORFs 1 and 2). ORF1 encodes a protein with a Mr 241,768 (242K) and ORF2, in a different frame within ORF1, encodes a Mr 36,179 (36K) protein. The number of nucleotides and the arrangement of ORFs of the isolate Li-23 genome are in perfect agreement with those of isolate P-209 from apple and isolate L from lily. Identities of the nucleotide sequence were 98.4% (Li-23/L) and 83.0% (Li-23/P-209) in whole genomes excluding poly(A) tail. A full-length cDNA clone (pCT35SF) of the isolate Li-23 genome was cloned into a plasmid vector containing cauliflower mosaic virus 35S RNA promoter which was designed to transcribe the isolate Li-23 genome from its first nucleotide. Mechanical inoculation of pCT35SF onto Chenopodium quinoa induced symptoms typical of isolate Li-23 infection. Virus particles, viral RNA and coat protein could be detected in pCT35SF-inoculated plants, demonstrating that pCT35SF is infectious