A xanthene-based novel colorimetric and fluorometric chemosensor for the detection of hydrazine and its application in the bio-imaging of live cells

• Published in: New journal of chemistry Vol. 45; no. 35; pp. 15869 - 15875
• Main Authors: Guria, Uday Narayan, Manna, Saikat Kumar, Maiti, Kalipada, Samanta, Sandip Kumar, Ghosh, Aritri, Datta, Pallab, Mandal, Debasish, Mahapatra, Ajit Kumar
• Format: Journal Article
• Language: English
• Published: CAMBRIDGE Royal Soc Chemistry 21.09.2021; Royal Society of Chemistry
• Subjects: Aqueous solutions; Biomedical materials; Charge transfer; Chemical sensors; Chemistry; Chemistry, Multidisciplinary; Chemoreceptors; Colorimetry; Cost analysis; Crystallography; Fluorescent indicators; Hydrazines; Indene; Physical Sciences; Science & Technology; Selectivity; OXIDATION; GOLD; SELECTIVE DETECTION; METHYLHYDRAZINE; EXPOSURE; RATIOMETRIC FLUORESCENT-PROBE
• ISSN: 1144-0546; 1369-9261
• DOI: 10.1039/d1nj02943f

Efficient reactive fluorescent chemosensors are generating considerable attention for analyte detection due to easy instrumentation, low cost and quick response. Hydrazine is a reactive chemical substance to the industry and is toxic to human health. Herein, an ICT-based hydrazine-responsive chemodosimeter 2-{(7,8-dihydro-3-methoxy-6 H -xanthen-5-yl)methylene}-2 H -indene-1,3-dione ( MXI ) sensor with high selectivity and quick response has been designed, synthesized and characterized. Upon treatment with hydrazine, the probe displays a distinct colour change to the naked eye, from violet to colourless in DMSO aqueous solution and a turn-on fluorescent signal is achieved. DFT calculations reveal that the hypsochromic shift in absorbance spectra and fluorescent enhancement was due to ICT (intramolecular charge transfer) blocking upon reaction with hydrazine and the probe. By its successful demonstrations in real-time test kit sensing, this probe overcomes most of the drawbacks of the fluorescent probes of recent times. Furthermore, this probe has also been effectively utilized for imaging intracellular hydrazine in human bone osteosarcoma (MG63) cells. An efficient xanthene-based colorimetric and fluorometric probe ( MXI ) for the selective detection of hydrazine in solution and in living cells.