The role of IκBα in TNF-α-induced apoptosis in hepatic stellate cell line HSC-T6

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 27; no. 4; pp. 407 - 410
• Main Authors: Qu, Zhijun, Lou, Duande, Pan, Yanfeng
• Format: Journal Article
• Language: English
• Published: Department of Infectious Diseases, Longgang Central District Hospital, Shenzhen 518116, China%Department of Infectious Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China%Department Infectious Diseases, the FirstAffiliated Hospital of Henan Medical University, Zhengzhou 450022, China 01.08.2007
• Subjects: nuclear factor-κB; apoptosis; hepatic stellate cells; nuclear factor inhibiting protein; tumor necrosis factor-α
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-007-0414-5

R3; To investigate the role of NF-κB in TNF-α induced apoptosis in HSC-T6, a mutant IκBα was transfected into HSC-T6 cells by lipofectin transfection technique and its transient effect was examined 48 h after the transfection. The activation of NF-κB was detected by immune fluorescence cytochemistry and Western blotting with anti-p65 antibody. The apoptosis and the rate of inhibition by TNF-α in both transfected and untransfected HSC-T6 cells were measured respectively by FAC-Scan side scatter analysis and MTT methods. Our results showed that TNF-α could activate NF-κB in untransfected cells but not in transfected HSC-T6 cells. The percentage of apoptosis in transfected cells were significantly higher than that in the untransfected ones (P＜0.01) and it was also true of the inhibition rate (P＜0.01). It is concluded that the resistance of HSC-T6 towards apoptosis induced by TNF-α can be mediated by NF-κB activation. The inhibition of NF-κB activation by mutant Iκ Bα can attenuate the resistance of HSC-T6 cells and increase its sensitivity to TNF-α.