Rubbing-assisted approach for highly-oriented collagen fibril arrays involving calcium phosphate precipitation

Highly-oriented homogeneous collagen (Col) fibril arrays were successfully fabricated on a rubbed polyimide (PI) film for precipitating calcium phosphate (CP). The surface functional groups of PI were aligned using the rubbing treatment, where the C&z.dbd;O bonds in imide groups had transition m...

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Published inMaterials chemistry frontiers Vol. 5; no. 1; pp. 3936 - 3948
Main Authors Chai, Yadong, Okuda, Mitsuhiro, Miyata, Mari, Liu, Zizhen, Tagaya, Motohiro
Format Journal Article
LanguageEnglish
Published London Royal Society of Chemistry 21.05.2021
Subjects
Absorption spectra
Arrays
Aspect ratio
Atomic force microscopes
Benzene
Biomedical materials
Biomimetics
Body fluids
Calcium ions
Calcium phosphates
Chemical composition
Collagen
Crystals
Fluorescence
Functional groups
In vitro methods and tests
Infrared spectroscopy
Ion adsorption
Nucleation
Polystyrene resins
Raman spectra
Rubbing
Self-assembly
Spin coating
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ISSN2052-1537
2052-1537
DOI10.1039/d1qm00114k

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Summary:Highly-oriented homogeneous collagen (Col) fibril arrays were successfully fabricated on a rubbed polyimide (PI) film for precipitating calcium phosphate (CP). The surface functional groups of PI were aligned using the rubbing treatment, where the C&z.dbd;O bonds in imide groups had transition moments perpendicular to the rubbing direction and the C&z.dbd;C (benzene rings), C-N and C-O-C bonds had transition moments parallel to the rubbing direction. After the Col molecular solution was spin-coated and self-assembled on the rubbed PI film, the Col fibrils parallel to the rubbing direction with an orientation ratio of 68 to 91% and a density of 121 ± 7 to 225 ± 22 number per mm were successfully formed on the rubbed PI film, indicating the occurrence of the interfacial interactions between the PI and Col molecules. Moreover, the carboxyl groups (-COOH) on the Col fibrils were used as the CP nucleation sites for Ca 2+ ion adsorption, and subsequently the nucleation of CP was promoted by immersing the Col fibrils into a simulated body fluid. Specifically, the CP crystals were effectively precipitated on the Col fibrils and plate-like CP crystals were formed along the direction of Col fibril arrays. Therefore, this provides an effective method for the fabrication of one-directional Col fibril arrays as well as CP/Col fibrous nanostructures using a biomimetic process. Highly-ordered homogeneous collagen fibril arrays were successfully fabricated on a rubbed polyimide film for precipitating calcium phosphate.
Bibliography:axis sizes, and the aspect ratio
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10.1039/d1qm00114k
2+
of CP crystals precipitated on PI-Col/CP-24 h and PI-Rub-Col/CP-24 h (Fig. S14). See DOI
ion and Col fibril (Fig. S11); GD-OES chemical composition profiles of PI-Rub-Col/CP-12 h, and illustration of the precipitation of CP on the hole zone of Col fibril by immersing into 1.5SBF (Fig. S12); XRD patterns of PI-Col/3, PI-Col/CP-12 h, PI-Col/CP-24 h, PI-Rub-Col/3, PI-Rub-Col/CP-12 h, and PI-Rub-Col/CP-24 h (Fig. S13); the short
and long
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Electronic supplementary information (ESI) available: Illustration of the polarized FT-IR measurement systems (Fig. S1); calculation method of O.R. values of Col fibrils on the PI and PI-Rub (Fig. S2); UV-vis spectra of the PI and PI-Rub films (Fig. S3); representative AFM topographic image of a PI-Rub film (Fig. S4); fluorescence microscope images of PI-CM/3, PI-CM/9, PI-AM/3, PI-AM/9, PI-Rub-CM/3, PI-Rub-CM/9, PI-Rub-AM/3, and PI-Rub-AM/9 (Fig. S5); polarized UV-vis spectral changes of PI-AM/3 and PI-Rub-AM/3 films (Fig. S6); illustration of the interfacial interaction mechanism between amino-modified fluorescent poly(styrene) particles and PI-Rub film (Fig. S7); Raman spectra of PI-Rub and PI-Rub-Col/3 (Fig. S8); absorption band assignments of the FT-IR spectra of PI-Col/3 and PI-Rub-Col/3 (Table S1); illustration of the highly-oriented processes of the Col fibrils on PI-Rub film (Fig. S9); FT-IR spectral deconvolution results of the amide I bands of Col fibrils from PI-Col/3 and PI-Rub-Col/3 (Fig. S10); FT-IR spectra of PI-Col/3, PI-Rub-Col/3, PI-Col/CP-12 h and PI-Rub-Col/CP-12 h, and illustration of the possible interfacial structure between Ca
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ISSN:2052-1537
2052-1537
DOI:10.1039/d1qm00114k