Diaminobenzidine cytochemistry in cryo-ultramicrotomy for the detection of peroxidases

Rat erythrocytes and lymphoid cells were fixed by glutaraldehyde and encapsulated in bovine serum albumin plus rabbit IgG globulins for cryo-ultramicrotomy. A technical procedure is described by which endogenous peroxidases of erythrocytes in ultrathin frozen sections were detected by hydrogen perox...

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Bibliographic Details
Published inHistochemistry (Berlin) Vol. 54; no. 4; p. 331
Main Authors Kuhlmann, W D, Viron, A
Format Journal Article
LanguageEnglish
Published Germany 28.12.1977
Subjects
3,3'-Diaminobenzidine
Animals
Benzidines
Fluorescent Antibody Technique
Frozen Sections
Histocytochemistry - methods
Immunoglobulin G
In Vitro Techniques
Lymph Nodes - ultrastructure
Peroxidases - analysis
Rats
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Summary:Rat erythrocytes and lymphoid cells were fixed by glutaraldehyde and encapsulated in bovine serum albumin plus rabbit IgG globulins for cryo-ultramicrotomy. A technical procedure is described by which endogenous peroxidases of erythrocytes in ultrathin frozen sections were detected by hydrogen peroxide and diaminobenzidine as hydrogen donor. Modifications of this classical cytochemical procedure proved also useful in cryoultramicrotomy for immunoperoxidase labeling of antigenic determinants in rabbit IgG globulins which have been crosslinked within the supporting matrix of cells.
ISSN:0301-5564
DOI:10.1007/BF00508275