A method for increasing the surface details of resin embedded viruses using congo/crimean haemorrhagic fever virus as a model

The surface details of resin embedded viruses can be greatly enhanced by the use of uranyl acetate block staining. The virions are embedded in agar immediately after fixation and block stained with 3% uranyl acetate in methanol during dehydration. This technique allows those particles outside their...

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Bibliographic Details
Published inJournal of virological methods Vol. 8; no. 1; pp. 57 - 61
Main Authors Ellis, D.S., Smith, M.D., Lloyd, G., Bowen, E.T.W., Simpson, D.I.H.
Format Journal Article
LanguageEnglish
Published London Elsevier B.V 01.02.1984
Amsterdam Elsevier
New York, NY
Subjects
Biological and medical sciences
Bunyaviridae - ultrastructure
electron microscopy staining
Fundamental and applied biological sciences. Psychology
Hemorrhagic Fever Virus, Crimean-Congo - ultrastructure
Microbiological Techniques
Microbiology
Microscopy, Electron
Organometallic Compounds
Staining and Labeling - methods
Techniques used in virology
Uranium
Virology
virus visualisation
virus visualisation
electron microscopy staining
Negative staining
Transmission electron microscopy
Method
Resins
Ultrastructure
Crimean-Congo hemorrhagic fever virus
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Summary:The surface details of resin embedded viruses can be greatly enhanced by the use of uranyl acetate block staining. The virions are embedded in agar immediately after fixation and block stained with 3% uranyl acetate in methanol during dehydration. This technique allows those particles outside their host cells to show clearly the spikes normally seen only by the separate use of negative staining.
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ISSN:0166-0934
1879-0984
DOI:10.1016/0166-0934(84)90040-5