The influence of culture medium composition on the incidence of chromosomal breakage

In patients with chromosomal instability and healthy subjects, significant differences were observed in the chromosomal breakage incidence in simultaneous lymphocyte cultures set up with TC medium 199, Eagle's Minimum Essential Medium, or RPMI, or RPMI 1629. The importance of the choice of cult...

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Bibliographic Details
Published inHuman genetics Vol. 50; no. 3; p. 277
Main Authors Keck, M, Emerit, I
Format Journal Article
LanguageEnglish
Published Germany 01.09.1979
Subjects
Ascorbic Acid
Cells, Cultured
Chromosome Aberrations - pathology
Chromosome Disorders
Chromosomes, Human - ultrastructure
Culture Media
Cysteine
Humans
Lymphocytes - physiology
Polysorbates
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Summary:In patients with chromosomal instability and healthy subjects, significant differences were observed in the chromosomal breakage incidence in simultaneous lymphocyte cultures set up with TC medium 199, Eagle's Minimum Essential Medium, or RPMI, or RPMI 1629. The importance of the choice of culture medium for mutagenicity testing and studies of so-called spontaneous breakage is shown. Cultures incubated with TC Medium 199 showed the highest chromosomal breakage incidence.
ISSN:0340-6717
DOI:10.1007/BF00399393