Comparison of a coagglutination test with the GM1-ELISA and the Y-1 adrenal cell assay for the detection of heat-labile enterotoxin from Escherichia coli

• Published in: Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology Vol. 260; no. 4; p. 436
• Main Authors: Steinrück, H, Samtleben, S, Rönnberg, B, Wadström, T
• Format: Journal Article
• Language: English
• Published: Germany 01.12.1985
• Subjects: Adrenal Glands; Agglutination Tests; Animals; Bacterial Toxins - analysis; Bacterial Toxins - toxicity; Cell Line; Cell Survival - drug effects; Enterotoxins - analysis; Enterotoxins - toxicity; Enzyme-Linked Immunosorbent Assay; Escherichia coli - analysis; Escherichia coli Proteins; G(M1) Ganglioside; Humans; Species Specificity
• ISSN: 0176-6724
• DOI: 10.1016/S0176-6724(85)80064-X

A number of different assay methods has been developed for the detection of the heat-labile enterotoxin (LT) of ETEC strains isolated from humans and animals. In the present study 40 Escherichia coli strains were subject of a comparative study of the staphylococcal coagglutination (Coa-LT) test, the GM1-ELISA and the Y-1 adrenal cell test. All but two of 20 "LT-ST" or "LT-only" producing ETEC strains gave identical results in both the Coa-LT test and the bioassay. None of the 14 "ST only" producing ETEC strains gave false positive results. It is concluded that the Coa-LT test is a simple and rapid assay method for the routine diagnosis of LT producing ETEC strains.