The role of β1 integrin subfamily in anchorage-dependent apoptosis of breast carcinoma cells differing in multidrug resistance

• Published in: Biochemistry (Moscow) Vol. 71; no. 5; pp. 489 - 495
• Main Authors: Morozevich, G. E., Kozlova, N. I., Preobrazhenskaya, M. E., Ushakova, N. A., Eltsov, I. A., Shtil, A. A., Berman, A. E.
• Format: Journal Article
• Language: English
• Published: New York Springer 01.05.2006; Springer Nature B.V
• Subjects: Adenocarcinoma; Anchorages; Anoikis; Antibodies; Apoptosis; Breast carcinoma; Drug resistance; Extracellular matrix; Integrins; Invasiveness; Metastasis; Multidrug resistance; Properties; Signaling; Russia
• ISSN: 0006-2979; 1608-3040
• DOI: 10.1134/S000629790605004X

Integrin expression was investigated in MCF-7 human breast adenocarcinoma line and in the MCF-7Dox line, which was selected from MCF-7 by a resistance to multiple antitumor drugs (MDR). We have shown that acquisition of MDR was accompanied by a drastically reduced expression of some integrins of the β1-subfamily (α2β1, α3β1, α6β1) and of αvβ5 intergin in the adenocarcinoma cells. In contrast, expression of α5β1 integrin was markedly increased in the MDR cells. Along with multiple antitumor drug resistance, MCF-7Dox cells demonstrate elevated resistance to anchorage-dependent apoptosis (anoikis) and enhanced in vitro invasive activity. To elucidate the implication of β1-integrins in the above phenotypic modifications, the effect of β1-integrin signaling was assayed. Stimulation of β1-mediated signaling was accomplished by treating of the cells with antibodies to the β1-subunit common for members of the β1-subfamily. These data show that activation of β1-integrin signaling markedly upregulated anoikis of the adenocarcinoma cells.