The importance of individual nucleotides for the structure and function of rRNA molecules in E. coli: A mutagenesis study

• Published in: FEBS Letters [FEBS LETT.]. Vol. 204, no. 1. 1986 Vol. 204; no. 1; pp. 89 - 95
• Main Authors: Meier, N., Göringer, H.U., Kleuvers, B., Scheibe, U., Eberle, J., Szymkowiak, C., Zacharias, M., Wagner, R.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 11.08.1986; Elsevier
• Subjects: Base Sequence; Biological and medical sciences; Escherichia coli; Escherichia coli - genetics; Fundamental and applied biological sciences. Psychology; Molecular and cellular biology; Molecular genetics; Mutagenesis; Mutation; Plasmids; Poly U - metabolism; Protein-RNA interaction; Proteins - metabolism; ribosomal RNA; Ribosomes - analysis; RNA, Ribosomal - analysis; RNA, Transfer - analysis; RNA, Transfer - metabolism; RNA, Transfer, Amino Acyl - metabolism; Structure-Activity Relationship; Subunit association; Translation. Translation factors. Protein processing; tRNA binding; Subunit association; ribosomal RNA; Mutagenesis; tRNA binding; Protein-RNA interaction; Translation; 5S-»RNA; Escherichia coli; Molecular interaction; Subunit; Ribosomal protein; Messenger RNA; Gene; Ribosomal RNA; T RNA; Molecular association; 16S-RNA; Bacteria; Affinity; Mutation; Escherichieae; Enterobacteriaceae
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(86)81392-8

Methods of in vitro mutagenesis were employed to determine the importance of individual nucleotides within the ribosomal RNAs for the structure and function of E. coli ribosomes. A series of denned nucleotides in the genes for the 5 S and 16 S RNA were altered by transition and transversion mutations using either oligonucleotide-directed or bisulfite-catalyzed mutation procedures. Plasmids harbouring the mutated rRNA genes were expressed and the ribosomes containing such altered RNAs were investigated for impairments in RNA-protein interaction assembly and mRNA-coded tRNA binding.