Technique to assess gene function in hop (Humulus lupulus L.) - powdery mildew interactions

Improving resistance to powdery mildew (Podosphaera macularis ssp. humuli, Pm) is a major objective in hop breeding. To achieve this, classical breeding can be assisted by marker-assisted breeding or by transgenic approaches. For both these techniques, it would be of advantage to have DNA sequences...

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Bibliographic Details
Published inActa horticulturae no. 1010; pp. 59 - 66
Main Authors Oberhollenzer, K, Seigner, E, Eichmann, R, Hückelhoven, R
Format Journal Article
LanguageEnglish
Published International Society for Horticultural Science 01.01.2013
Subjects
barley
DNA
gene silencing
genes
Humulus lupulus
marker-assisted selection
mildews
nucleotide sequences
Podosphaera
powdery mildew
wheat
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Summary:Improving resistance to powdery mildew (Podosphaera macularis ssp. humuli, Pm) is a major objective in hop breeding. To achieve this, classical breeding can be assisted by marker-assisted breeding or by transgenic approaches. For both these techniques, it would be of advantage to have DNA sequences available with confirmed function in the hop-powdery mildew interaction. Here, we report on the adaptation of a transient transformation assay for the functional assessment of defence- or susceptibility-related genes in hop as already available for barley and wheat. As a proof of concept, we performed transient-induced gene silencing (TIGS) of a putative hop powdery mildew susceptibility factor in hair cells, which resulted in a significantly reduced penetration success of the powdery mildew fungus.
Bibliography:http://www.actahort.org/books/1010/1010_6.htm
ISSN:0567-7572
DOI:10.17660/ActaHortic.2013.1010.6