β4GalT-II increases cisplatin-induced apoptosis in HeLa cells depending on its Golgi localization

• Published in: Biochemical and biophysical research communications Vol. 358; no. 1; pp. 41 - 46
• Main Authors: Jiang, Jianhai, Zhou, Jin, Wei, Yuanyan, Shen, Jialin, Liu, Dan, Chen, Xiaoning, Zhang, Si, Kong, Xiangfeig, Yun, Xiaojing, Gu, Jianxin
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 01.06.2007
• Subjects: Apoptosis; Cisplatin; Golgi localization; HeLa cells; β4GalT-II; HeLa cells; β4GalT-II; Golgi localization; Cisplatin; Apoptosis
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2007.04.044

β1,4-Galactosyltransferase II (β4GalT-II) is one of the enzymes transferring galactose to the terminal N-acetylglucosamine of complex-type N-glycans and its expression is significantly altered during oncogenesis with unknown functions. Here, we reported for the first time the pro-apoptotic role of β4GalT-II in tumor cells. The level of β4GalT-II mRNA expression was obviously decreased during HeLa cell apoptosis induced by cisplatin. Interestingly, the ectopic expression of β4GalT-II in HeLa cells markedly increased apoptosis and cleavage of PARP induced by cisplatin as well as the expression of pro-apoptotic protein Bax. Furthermore, deletion of Golgi localization domain abolished the apoptotic role of β4GalT-II in HeLa cells. Collectively, these results suggest that β4GalT-II increases HeLa cell apoptosis induced by cisplatin depending on its Golgi localization, which indicates that β4GalT-II might contribute to the therapeutic efficiency of cisplatin for cervix cancer.