Conditional hyporecombination mutants of three REC genes of Saccharomyces cerevisiae

We have isolated and characterized three conditional hyporecombination mutants, rec1-1, rec3-1 and rec4-1, that define three REC genes of Saccharomyces cerevisiae required for spontaneous general mitotic interchromosomal recombination. Each MATa/MAT alpha rec/rec diploid is deficient in mitotic sing...

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Bibliographic Details
Published inCurrent genetics Vol. 17; no. 1; pp. 7 - 12
Main Authors Esposito, M.S, Brown, J.T
Format Journal Article
LanguageEnglish
Published United States 1990
Subjects
Chromosome Mapping
Chromosomes, Fungal
Diploidy
Gene Conversion
genes
Genes, Fungal
Genetic Complementation Test
genetic recombination
mutants
Mutation
Phenotype
Recombination, Genetic
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - physiology
Spores, Fungal
sporulation
Temperature
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Summary:We have isolated and characterized three conditional hyporecombination mutants, rec1-1, rec3-1 and rec4-1, that define three REC genes of Saccharomyces cerevisiae required for spontaneous general mitotic interchromosomal recombination. Each MATa/MAT alpha rec/rec diploid is deficient in mitotic single site gene conversion, intragenic recombination, intergenic recombination and sporulation at the restrictive temperature (36 degrees C). The rec1-1 mutation also confers conditional enhanced sensitivity to the killing effects of X-rays. The rec1-1 and rec3-1 mutations have been mapped to chromosome VII. The rec1-1, rec3-1 and rec4-1 mutations exhibit complementation at 36 degrees C for both mitotic recombination and sporulation.
ISSN:0172-8083
1432-0983
DOI:10.1007/BF00313242