Coordination changes and auto-hydroxylation of FIH-1: Uncoupled O2-activation in a human hypoxia sensor

• Published in: Journal of inorganic biochemistry Vol. 102; no. 12; pp. 2120 - 2129
• Main Authors: Chen, Yuan-Han, Comeaux, Lindsay M., Herbst, Robert W., Saban, Evren, Kennedy, David C., Maroney, Michael J., Knapp, Michael J.
• Format: Journal Article
• Language: English
• Published: United States 01.12.2008
• Subjects: Anaerobiosis; Basic Helix-Loop-Helix Transcription Factors - metabolism; Catalytic Domain; Ferrous Compounds - chemistry; Ferrous Compounds - metabolism; Humans; Hydroxylation; Iron - metabolism; Mixed Function Oxygenases; Oxygen - metabolism; Recombinant Proteins - chemistry; Recombinant Proteins - isolation & purification; Recombinant Proteins - metabolism; Repressor Proteins - chemistry; Repressor Proteins - isolation & purification; Repressor Proteins - metabolism

Hypoxia sensing is the generic term for pO2-sensing in humans and other higher organisms. These cellular responses to pO2 are largely controlled by enzymes that belong to the Fe(II) alpha-ketoglutarate (alphaKG) dependent dioxygenase superfamily, including the human enzyme called the factor inhibiti...