Chemical study of Adenocalymma axillarum crude leaf extract and isolated compounds

Abstract Adenocalymma axillarum (K.Schum.) L.G. Lohmann is a liana belonging to the family Bignoniaceae. In traditional medicine, the genus Adenocalymma is used to treat fever, skin ailments, and body, joint, and facial muscle pains, and it is also applied as cosmetic. Biological assays conducted wi...

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Published inBrazilian Journal of Pharmaceutical Sciences Vol. 58
Main Authors Andrade, João Paulo Campos, Alves, Osvaine Junior Alvarenga, Costa, Marian Caroline, Gimenez, Valéria Maria Melleiro, Squarisi, Iara Silva, Nicolella, Heloiza Diniz, Pagotti, Mariana Cintra, Tavares, Denise Crispim, Cunha, Wilson Roberto, Silva, Márcio Luís Andrade e, Januario, Ana Helena, Magalhães, Lizandra Guidi, Pauletti, Patrícia Mendonça
Format Journal Article
LanguageEnglish
Published Universidade de São Paulo, Faculdade de Ciências Farmacêuticas 01.01.2022
Universidade de São Paulo
Subjects
Cytotoxicity
Flavonol
HPLC-HRMS-DAD
Iridoid
Leishmanicidal
PHARMACOLOGY & PHARMACY
Iridoid
Cytotoxicity
Leishmanicidal
HPLC-HRMS-DAD
Flavonol
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Summary:Abstract Adenocalymma axillarum (K.Schum.) L.G. Lohmann is a liana belonging to the family Bignoniaceae. In traditional medicine, the genus Adenocalymma is used to treat fever, skin ailments, and body, joint, and facial muscle pains, and it is also applied as cosmetic. Biological assays conducted with the A. axillarum crude leaf ethanol extract have indicated leishmanicidal activity and absence of cytotoxicity. This study aimed to analyze the A. axillarum leaf ethanol crude extract by high-performance liquid chromatography-high-resolution mass spectrometry- diode array detector (HPLC-HRMS-DAD) and to evaluate the leishmanicidal and cytotoxic activities of this crude extract, its fractions, and isolated compounds. HPLC-HRMS-DAD analysis of this extract revealed that it consisted mainly of flavonoids, with nine major compounds. Extract purification yielded 4-hydroxy-N-methylproline, 6-β-hydroxyipolamiide, quercetin-3-O-robinobioside, hyperin, isorhamnetin-3-O-robinobioside, and 3’-O-methylhyperin, which were identified by Nuclear Magnetic Resonance. The isolated compounds were inactive against Leishmania amazonensis promastigotes and human lung fibroblast cells.
ISSN:2175-9790
2175-9790
DOI:10.1590/s2175-97902022e20278